SLIT2 and glioblastoma: Furthermore, co-culture of siRobo1/MDA-MB-231 cells (upper chamber) with brain derived cells (glioblastoma cell line LN229, lower chamber) were performed in the transwell system, we found LN229 cells with 100 ng/ml of Slit2-N in the lower chamber promoted the migration of siRobo1 cells compared with the Slit2-N absent group (Student’s t test, P = 0.013, Fig. 4d).