To create a system to study the effect of G6PD S84 glycosylation on cellular metabolism, we depleted endogenous G6PD and stably expressed small hairpin RNA (shRNA)-resistant Flag-tagged WT or S84V G6PD in A549 lung cancer cells (henceforth referred to as WT G6PD or S84V G6PD replacement cells, Supplementary Fig. 5). This evidence concerns the gene G6PD and lung carcinoma.