To gain a better understanding of the impact of G6PD glycosylation on cancer development, we first examined whether G6PD is glycosylated in different human solid tumor cells, including breast cancer cell line, MCF7, lung cancer cell lines, H661 and A549, ovarian cancer cell line, SKOV-3, melanoma cell line, A375, and osteosarcoma cell line, U2OS (Supplementary Fig. 11). This evidence concerns the gene G6PD and osteosarcoma.