Following validations in vitro, 5.0×105 (trans-hEN OTX2 knockdown and trans-hEN scramble) and 2.5×105 (D283 OTX2 knockdown and D283 scramble) cells were injected into the right frontal lobe (n=5 for each condition and cell line) of NOD SCID mice and examined for tumor formation after 45 days. The gene discussed is OTX2; the disease is neoplasm.