Among the 16754 single patients tested for ANA, 421 patients (2.5%) had anti-DFS70 antibodies (Table 1); 83% were females (n = 348, female/male sex ratio 4.8), 52 patients had SLE (12.4%), 9, RA (2.1%), 15, primary Sjögren syndrome (3.6%), 2, inflammatory myositis (0.5%) and 2 had mixed connective tissue diseases (0.5%). This evidence concerns the gene BTG3 and mixed connective tissue disease.