The SEMA5A regulatory network includes previous ASD candidate genes and regions including MACROD2, CDH8, FOXP1, AUTS2, MBD5, among others.23 Third, two independent studies have reported a reduced level of SEMA5A mRNA in Epstein–Barr virus-transformed B lymphocytes22 or in the brains of patients with ASD.20 Finally, SEMA5A is located on chromosome 5 in a minimal region deleted in patients with ‘cri-du chat' syndrome. This evidence concerns the gene AUTS2 and Cri-du-chat syndrome.