To date, few research papers have reported its mutant, whereas the modifications and regulations are critical for HMGB1 location and function: Acetylated HMGB1 in cancer cells participates DNA replication; ADP-ribosylation of HMGB1 regulates cell death; methylation of HMGB1 facilitates its translocation from nucleus to the cytoplasm; phosphorylation of HMGB1 affects both its DNA-binding activity and nucleo-cytoplasmic distribution and release. Here, HMGB1 is linked to cancer.