We found that in cells expressing both the TLR4 co-receptors MD2 and CD14 (MDA-MB-231 cells; Fig. 5c left) TLR4-GFP was expressed in a vesicular pattern in the cytosol, whereas in breast cancer cells lacking both MD2 and CD14 (MCF-7 cells; Fig. 5c right), TLR4-GFP was expressed evenly in the cytoplasm. This evidence concerns the gene LY96 and breast cancer.