Apoptosis in MDA-MB-231 breast cancer cells where TLR2 or TLR4 was silenced gave either conflicting results (TLR2) or was not affected (TLR4) (Additional file 2: Figure S1A), and finally proliferation using 3H-incorporation assays of MDA-MB-231 breast cancer cells where TLR4 was silenced was not affected as compared to control (Additional file 2: Figure S1B). This evidence concerns the gene TLR4 and breast cancer.