To date, this is the first report focusing on anti-virulence activity of ACP, which clearly demonstrated that, although there was no antibiofilm effect observed against P. aeruginosa at the parameters examined, the treatment exhibited high potential against QS-regulated virulence factors and these observations may further serve as a means for exploration of new ACP-based strategies for tackling infections caused by this microorganism. Here, NDUFAB1 is linked to infection.