As depicted in Fig 3, ROC curve analysis displayed an area under the curve for the diagnosis of CKD stage 3 or higher of 0.88 (0.80–0.95, p < 0.001) for total serum TFF1 and 0.94 (0.84–1, p < 0.001) for total serum TFF3 concentrations. The gene discussed is TFF3; the disease is chronic kidney disease.