Gli-1 mRNA level was increased in a number of breast cancer cell lines, including MDA-MB-453 (TN), MDA-MB-231 (TN and basal type B), BT20 (basal type A), MCF10A (benign breast cancer cell line) and SKBR3 (HER2+) in comparison to a primary human mammary epithelial cells (HMEC) [45]. This evidence concerns the gene ERBB2 and breast cancer.