To unravel a putative crosstalk between ASFV and these domains, indirect immunofluorescence analysis of Vero-infected cells was performed from 4 h post infection (hpi) onwards, using antibodies that recognize the major constituent protein of the studied subnuclear domains (anti-SC-35 to identify nuclear speckles, anti-coilin to label Cajal bodies and anti-PML to detect PML-NBs), and an anti-ASFV swine whole serum to label infected cells. This evidence concerns the gene PML and infection.