Although all studied nuclear HDACs (class I—HDAC1, HDAC2 and HDAC3) partially localized with the cytoplasmic viral factories, only HDAC2 which regulates chromatin plasticity, revealed an increased signal intensity in ASFV-infected cells, from an early phase of infection (Figure 4q–t), not detected in non-infected cells (Figure 4u–z). Here, HDAC2 is linked to infection.