AKT1 and infection: Infection with both viruses increased the relative luciferase activity via the ISRE regulatory element in luciferase assays, and both LY294002 (50 μM) treatment (Figure 7G) or knockdown of Akt (Figure 7H) eliminated virus-induced activation of ISRE, indicating that activated PI3K/Akt in reovirus infection triggered the ISRE via EMSY.