Consistent with the results in angiogenesis assay, the immunohistochemical analysis indicated that there were a rare numbers of CD31-positive microvessels from GSK-3β-expressing group compared with GFP control group, revealing that GSK-3β overexpression significantly reduced the formation of tumor microvessels (Figure 4C and 4D). The gene discussed is PECAM1; the disease is neoplasm.