In this study, we test the impact of FANCF, FANCL and/or FANCD2 knockdown by small interfering RNA (siRNA) on the FA/BRCA pathway function and the sensitivity to cisplatin in human lung cancer cells A549 (an adenocarcinoma cell line), A549/DDP (a cisplatin-resistant A549 cell line), and SK-MES-1 (a squamous cancer cell line). The gene discussed is FANCD2; the disease is lung cancer.