These mechanistic differences may also explain why HRV2 cleaves both isoforms at the same rate in infected HeLa cells whereas in HRV14 and PV (both of which are more closely related to CVB4 than HRV2) infected HeLa cells, the eIF4GI isoforms are cleaved more rapidly than the eIF4GII isoforms.35, 41, 42 Investigation of the importance of eIF4E interactions on the cleavage of full‐length eIF4G isoforms during infection awaits the development of cell lines over‐expressing recombinant eIF4G that can be infected by picornaviruses. Here, EIF4G1 is linked to infection.