HNF4A and ductal breast carcinoma in situ: As shown in Fig. 9b and c, KD of BCL9 significantly reduced β-catenin/TCF-mediated transcription (p <0.05) in DCIS.COM, both in the presence and absence of Wnt3A stimulation, compared to similarly treated NT and controls, but not in SUM225 cells with or without Wnt3a stimulation (data not shown).