As shown in Fig. 6a, BCL9 KD DCIS.COM and SUM225 showed a significant reduction in phosphoH3 compared to the control DCIS.COM and SUM225 (DCIS.COM = 3.25 ± 0.01 cells per 500 KD cells compared to 5.25 ± 0.25 cells per 500 control cells counted; SUM225 = 7.83 ± 0.01 cells per 500 KD cells compared to 21.5 ± 2.36 per 500 control cells counted; p <0.05). This evidence concerns the gene BCL9 and ductal breast carcinoma in situ.