SHOC2 and melanoma: Immunoblotting analyses showed that activities of ERKs and Akt were significantly reduced by Sur8 knockdown in all of the melanoma cell lines tested (SK-Mel5, SK-Mel28, A375, G361, and B16-F10) (Figure 6A), and Sur8 interacted with p110α, Ras, and Raf-1 in B16-F10 cells at the endogenous protein level (Supplementary Figure 8A).