We also performed biochemical fractionation in breast cancer cell lines using nuclear and cytoplasmic extracts and found ALK expression to be more pronounced in cytosolic fraction (Figure S1 in Additional file 2) and had comparatively more expression in the triple-negative cell line compared to the non-tumorigenic epithelial breast cell line; MCF12A (Figure S2 in Additional file 3). This evidence concerns the gene ALK and breast carcinoma.