MAPT and Alzheimer disease: To investigate whether MIF affects tau phosphorylation, we examined the levels of total tau (using the Tau5 antibody), non-phosphorylated tau (using the Tau1 antibody), and phosphorylated tau at several AD-related amino acid positions including Ser199, Thr205, Ser396, and Ser404 in the hippocampus and cerebral cortex of mice receiving ICV injection of STZ.