Among the numerous differences between these two human cell lines, there are the following: their methylation status [31], the highest expression of hepatocyte differentiating transcription factors CEBPA and HNF4A in HuH7 in comparison to HepG2 cells [32], and lack of the detoxifying enzyme COX2 in HepG2 cells [33] despite the fact that this enzyme is able to induce cell death in HCC cells [34]. Here, HNF4A is linked to hepatocellular carcinoma.