In the present study, we have established a sandwich ELISA using the L59 antibody against the N-terminus of the L59 LAP-DPs (Fig. 1) and successfully detected L59 LAP-DPs in both human HSC cultures (Fig. 3) and in two mouse hepatic fibrosis models (Figs. 4 and 5). The gene discussed is TGFB1; the disease is Hepatic fibrosis.