CD28 and acute graft versus host disease: Tregs were isolated from a third party UCB graft and expanded polyclonally with anti-CD3/CD28 coated beads and recombinant IL-2 over a period of 18 days. Patients received expanded Tregs at doses ranging from 1 × 105/kg to 30 × 105/kg. Targeted Treg dose was only achieved in 74% of cases. Compared with the 108 historical controls, there was a reduced incidence of grades II–IV acute GvHD (from 61–43%; p = 0.05), although the overall incidence of GvHD was not significantly different.