We have analyzed several available microarray gene expression datasets from ischemic and non-ischemic muscles in two mouse models of PAD (in C57BL/6 and BALB/c mouse species) from Hazarika et al. (2013) to identify important genes/proteins in PAD, such as THBS1 (thrombospondin-1), TLR4 (toll-like receptor 4), EphA4 (EPH receptor A4), and TSPAN7 (tetraspanin 7). This evidence concerns the gene TLR4 and peripheral arterial disease.