Further immunoblotting analysis revealed that in contrast to other cancer types, PME‐1 silencing did not inhibit expression of active phosphorylated forms of serine–threonine‐specific protein kinases AKT‐1/2/3 (p‐AKT) and ERK‐1/2 (p‐ERK) in either of the colon cancer cell lines (Fig. 3C and D). This evidence concerns the gene PPME1 and colonic neoplasm.