Using DNA isolated from formalin-fixed paraffin-embedded archival tissue, mutation status of TP53, APC, KRAS, PIK3CA, FBXW7, SMAD4, BRAF and NRAS was determined by targeted next generation sequencing, which succeeded for 204 CRC samples (Fig 2, S3 and S4 Tables). This evidence concerns the gene SMAD4 and colorectal carcinoma.