The results showed that high expression of Cripto-1 (P < 0.001), high AFP level (P < 0.001), high GGT level (P = 0.009), liver cirrhosis (P = 0.006), larger tumor size (P < 0.001) and vascular invasion (P < 0.001) were unfavourable predictors for OS of HCC patients. This evidence concerns the gene AFP and cirrhosis of liver.