By using an integrated approach combing GR ChIP-exo, AP1/NF-κB ChIP-seq and RNA-seq analysis in TNBC cells with analysis of multiple clinical breast cancer data sets comprising over 2,000 patients, we found that Dex-liganded GR binds to a precisely defined GRE, to directly regulate genes associated with drug resistance and unfavourable clinical characteristics and outcomes (Figs 2, 3, 4). This evidence concerns the gene NFKB1 and breast cancer.