KIAA1549 and pilocytic astrocytoma: We performed RT-PCR to validate the presence of a fusion, which was further confirmed by Sanger sequencing and FISH, which highlighted a fusion between the exon 16 of KIAA1549 and the exon 9 of BRAF. The presence of KIAA1549:BRAF fusion is found in 60% of pilocytic astrocytomas [16] and was not previously detected in RGNT in the literature [24–26].