Therefore, to evaluate whether EVE treatment was able to interfere with the liver extracellular microenvironment by inducing EMT in rat stellate cell (HSC) and human liver cancer cell lines (HepG2), we measured, by RT-PCR, changes in expression level of genes encoding for well known EMT markers (α-SMA, Fibronectin and Vimentin) after both low-therapeutic (10 nM) and high (100 nM) doses; low dose has been considered therapeutic as it corresponds to the usual serum range (3-8 ng/mL) in the liver transplant setting. This evidence concerns the gene ACTA1 and liver cancer.