As observed before infection, the ratios between IFN-γ/IL-4 and IFN-γ/IL-10 (Fig 6B), as well as between IL-12/IL-4 and IL-12/IL-10 (Fig 6C), using SLA to stimulate the cultures were calculated, and results showed that mice vaccinated with rLiHyp1/saponin, rLiHyp6/saponin, rHRF/saponin or polyproteins/saponin developed a parasite-specific Th1 response, after challenge infection. Here, IL4 is linked to infection.