In contrast, BCVs from macrophages infected with the mutant strains mCherry-ΔabcBA or mCherry-ΔvirB2 had a very low percentage of colocalization with calreticulin over course of infection, which coincided with the low percentage of macrophages containing intracellular bacteria (~5%; p<0.0001) (Fig 5C). Here, CALR is linked to infection.