CD4 and malaria: While the frequency of tetramer-positive CD4 T cells decreased in the liver, the absolute number of 2W1S-specific cells was actually higher during malaria infection (Fig 3A). This alteration was limited to the liver, since both the total number and frequency of 2W1S-specific CD4 T cells in spleen and lymph nodes (mesenteric and peripheral LN) was unchanged during active malaria infection (Figs 3A and S5). The reduced proportion (%) of 2W1S+ cells within the total T cell population during malaria may result from rapid influx of malaria-specific T cells in the liver.