DAG1 and type 1 diabetes mellitus: We then quantified mRNA expression for LAMP2, TBK1 and DAG1 in PB mononuclear cells (PBMCs) of children with recent-onset T1D (n=8), islet AB positive (n=11) and negative (n=18) first-degree relatives (FDRs) and unrelated healthy control children (n=12).