Immunofluorescence revealed co-expression of ANXA2 labeled by red fluorescence and HE4 labeled by green fluorescence in the cell membrane and cytoplasm of endometrial carcinoma cell lines (Fig. 2B) and different endometrial tissues (Fig. 3B), and the overlapping orange fluorescence observed at the expression sites suggested co-localization of ANXA2 and HE4. The gene discussed is ANXA2; the disease is endometrial carcinoma.