Based on the results of this study, we have raised a hypothesis that anethole might suppress TcpP and thereby virulence expression in V. cholerae by activating cAMP-CRP complex mediated signal, might have an impact to design new antibacterial compounds against multidrug resistant bacterial pathogens including V. cholerae. Although further studies are needed, our data suggest that anethole could be an antivirulence drug candidate against multiple antibiotic resistant toxigenic V. cholerae-mediated infections. Here, CRP is linked to infection.