Although our present data did not provide enough support to show the interaction between Twist1 and 4E-BP1, our results, which showed that increased 4E-BP1 transcription and decreased p-4E-BP1 after Twist1 knockdown while decreased 4E-BP1 transcription and increased p-4E-BP1 after Twist1 overexpression, indicate that Twist1′s pathophysiological feature in lung cancer could act through 4E-BP1 regulation. This evidence concerns the gene EIF4EBP1 and lung cancer.