CD27 and cancer: Human T cells virally transduced to express a folate receptor-α (FRα)-specific CAR, comprised of an extracellular murine anti-human FRα MOv-19 scFv and an intracellular CD3 zeta (CD3ζ) chain signaling module in tandem with a CD27 costimulatory endodomain displayed enhanced cytokine release, cytolytic function and proliferation in vivo, thereby rationalizing the incorporation of CD27 costimulation in CAR-T therapy for FRα+ cancer.