By KEGG analysis, the HOXA13-associated genes were involved in the TGF-β pathway, which is correlated to the EMT process; SMAD2 and SMAD3 were involved in a “Cancer Pathway.” Therefore, we further assayed SMAD2, SMAD3, and their respective phosphorylated forms by western blotting. The gene discussed is TGFB1; the disease is cancer.