To further verify the biological behavior of HOXA13 in gliomas, we constructed lentiviral vectors expressing nonsense control or HOXA13 siRNA, and subsequently infected the U87, U87-EGFRvIII (relatively high HOXA13 expression), LN229, and U251 (relatively low HOXA13 expression) cell lines with these lentiviruses. Here, HOXA13 is linked to central nervous system cancer.