ICC revealed specific staining for each of the positive markers of MSC stemness (CD105, CD90, CD44, CD29) while expression of the negative marker (CD45) was undetectable in Phase A cells (P+5) but increased to detectable levels in Phase C cells (P+13) (Fig 2; Table 3). This evidence concerns the gene ITGB1 and intrahepatic cholangiocarcinoma.