Inclusion criteria were: patients with unifocal ductal breast cancer, palpable, diameter less than < 3 cm histologically proven by evaluation ER, PgR, HER-2; absence of inflammatory features; good definition of lesion to ultrasound; distance between tumor margin and skin > 0.5 cm; no metastases; written informed consent. The gene discussed is ERBB2; the disease is breast carcinoma.