TP53 and hepatocellular carcinoma: Table 1 shows IC50 values of TCD in all HCC cell lines tested. All HCC cells were sensitive to TCD treatment with IC50 values ranging from 2–50 nM. Remarkably, Hep-J5 and Mahlavu cells showed higher responses to TCD, with IC50 values of approximately 10 nM. We also found that compared to TCD treatment (IC50<50 nM), higher IC50 values (of around 190 nM) of colchicine were obtained in the well-differentiated (Huh7) and p53-null (Hep-3B) HCC cell lines.