Under those conditions, melanoma cells were strongly sensitized to DL-mediated caspase-dependent (zVAD-fmk) (Figure 1b, black columns and Figure 1c) but RIPK1-independent (Nec-1), as shown by Annexin-V/propidium iodide (PI) double staining (Figure 1c), apoptosis when compared with cells treated with CD95L alone (Figure 1b, grey columns). Here, PCSK1 is linked to melanoma.