CASP1 and steatosis: Indeed, a deficiency in caspase-1, Nlrp3 or ASC in mice results in protection from high-fat diet (HFD)-induced steatosis and insulin resistance.16, 22, 23 Similarly, a deficiency in IL-1β, IL-1 Receptor or TLR424 protects mice from methionine- and choline-deficient (MCD) diet-induced steatohepatitis.