To further confirm glomerular Angptl4 expression in MCD, we stained for Angptl4, desmin and synaptopodin in samples obtained from nephrotic syndrome patients with MCD (n = 15), MN (n = 5), FSGS (n = 5) and mesangial proliferative glomerulonephritis (MsPGN, n = 5). This evidence concerns the gene ANGPTL4 and nephrotic syndrome.