As expected, ChIP and quantitative PCR (ChIP-qPCR) assays showed high levels of SMYD3 occupancy at the SMYD3 binding elements in mock-depleted bladder (J82 and T24)/colon (HCT116 and CaCO2) cancer cells, but SMYD3 levels were markedly decreased after SMYD3 knockdown (Supplementary Figure S5C). This evidence concerns the gene SMYD3 and cancer.