In a South African TB endemic population, we evaluated the serodiagnostic reactivity of L-alanine dehydrogenase (AlaDH) (Rv2780), nitrate/nitrite response transcriptional regulator NarL (Rv0844c), periplasmic phosphate-binding lipoprotein PstS3 (Rv0928), 19 kDa lipoprotein antigen precursor LpqH (Rv3763), and lipoprotein MPT83 (Rv2873). This evidence concerns the gene ALAD and tuberculosis.