EA preconditioning at ST36 obviously ameliorated CLP-induced intestinal injury and high permeability and exerted protective effects on intestinal mucosal immune barrier; EA preconditioning significantly increased the percentage of CD3+, γ/δ, and CD4+ T cells and the ratio of CD4+/CD8+ T cells and ultimately reduced the mortality of CLP-induced sepsis in rats. This evidence concerns the gene CD4 and Sepsis.