In another study, amplification and overexpression of the CTTN gene correlated with lymph node metastasis in ESCC, while in vitro analyses showed a role for Cortactin in anoikis resistance, tumor growth, and lung metastasis of ESCC cells via the PI3K/Akt pathway [69]. The gene discussed is AKT1; the disease is esophageal squamous cell carcinoma.