Compared to monoculture, transwell co-culture, and MSCs conditioned media treated breast cancer cells, patterned co-culture significantly (p < 0.05 and p < 0.01) increased Src phosphorylation at Y416 (Src-pY416) (Fig. 3B and Supplementary Fig. 5), an indicator of Src activity6, 7, 36. Here, SRC is linked to breast cancer.