IGF2 and malignant colon neoplasm: In conclusion, through bisulfite pyrosequencing and subsequent confirmation by nucleotide sequencing, we identified and validated a polymorphic variant that directly prevents methylation at one of the CpG dinucleotides that comprise the IGF2 DMR, a region for which hypomethylation has previously been associated with a compromised environment during prenatal development as well as risk of colon cancer.